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Background  Some types of flavonoid intermediates seemed to be restricted to plants. Naringenin is a typical plant metabolite, that has never been reported to be produced in prokariotes. Naringenin is formed by the action of a chalcone synthase using as starter 4-coumaroyl-CoA, which in dicotyledonous plants derives from phenylalanine by the action of a phenylalanine ammonia lyase.    Results  A compound produced by  Streptomyces clavuligerus  has been identified by LC–MS and NMR as naringenin and coelutes in HPLC with a naringenin standard. Genome mining of  S. clavuligerus  revealed the presence of a gene for a chalcone synthase ( ncs ), side by side to a gene encoding a P450 cytochrome ( ncyP ) and separated from a gene encoding a Pal/Tal ammonia lyase ( tal ). Deletion of any of these genes results in naringenin non producer mutants. Complementation with the deleted gene restores naringenin production in the transformants. Furthermore, naringenin production increases in cultures supplemented with phenylalanine or tyrosine.    Conclusion  This is the first time that naringenin is reported to be produced naturally in a prokariote. Interestingly three non-clustered genes are involved in naringenin production, which is unusual for secondary metabolites. A tentative pathway for naringenin biosynthesis has been proposed.    Electronic supplementary material  The online version of this article (doi:10.1186/s12934-015-0373-7) contains supplementary material, which is available to authorized users.

Molecular genetics of naringenin biosynthesis, a typical plant secondary metabolite produced by Streptomyces clavuligerus