English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  Sugar alcohols have been widely applied in the fields of food and medicine owing to their unique properties. Compared to chemical production, microbial production of sugar alcohols has become attractive because of its environmentally friendly and sustainable characteristics. Our previous study identified the nonconventional yeast  Pichia anomala  TIB-x229 as a potential producer of sugar alcohols from glucose. To further improve strain performance, we combined genome shuffling with optimized high throughput screening methods for the directed improvement of nonconventional yeast and complex phenotypes.    Results  To accelerate strain improvement, a practical genome shuffling procedure was developed and successfully applied in the nonconventional yeast  P. anomala  to increase sugar alcohol production. Through two rounds of genome shuffling, an improved  P. anomala  isolate GS2-3 could produce 47.1 g/L total sugar alcohols from 100 g/L glucose, which was 32.3% higher than the original strain. In this process, a simple and accurate colorimetric assay was optimized and used for high throughput screening of sugar alcohol-producing strains. Moreover, a fluorescence-activated cell sorting method was developed to efficiently screen protoplast fusions for genome shuffling of nonconventional yeast.    Conclusion  An efficient genome shuffling procedure was developed and applied to enhance the sugar alcohol production of the nonconventional yeast  P. anomala . Our results provide a general platform for strain improvement of polyol-producing microorganisms or nonconventional microorganisms in the future.    Electronic supplementary material  The online version of this article (doi:10.1186/s12934-015-0303-8) contains supplementary material, which is available to authorized users.

Genome shuffling of the nonconventional yeast Pichia anomala for improved sugar alcohol production