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Background   Saccharomyces cerevisiae  is the micro-organism of choice for the conversion of fermentable sugars during beverage or bioethanol fermentations. These fermentations are characterised by high osmotic stress on a yeast cell, with selected brewing fermentations beginning at 20–25% fermentable sugars and bioethanol fermentations at 13% fermentable sugars.    Results   RCK2  encodes for a MAPKAP (MAPK-activated protein kinase) enzyme and was identified on a locus by QTL analysis in yeast cells under osmotic stress,  RCK2  expression was placed under a tetracycline regulatable vector and rescued glucose, sorbitol or glycerol induced osmotic stress in an  rck2  null strain. A strain overexpressing  RCK2  had significantly faster fermentation rates when compared with the empty vector control strain.    Conclusions  Presence of  RCK2  increased rates of glucose utilisation (~40 g glucose in first 8 h) during a 15% glucose fermentation and concurrent production of ethanol when compared with empty vector controls. Tolerance to osmotic stress using the tetracycline regulatable vectors could be turned off with the addition of tetracycline returning a  rck2  null strain back to osmotic sensitivity.    Electronic supplementary material  The online version of this article (doi:10.1186/s12934-015-0276-7) contains supplementary material, which is available to authorized users.

Expression of RCK2 MAPKAP (MAPK-activated protein kinase) rescues yeast cells sensitivity to osmotic stress