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Background  Inosine and guanosine monophosphate nucleotides are convenient sources of the umami flavor, with attributed beneficial health effects that have renewed commercial interest in nucleotide fermentations. Accordingly, several bacterial strains that excrete high levels of inosine and guanosine nucleosides are currently used in the food industry for this purpose.    Results  In the present study, we show that the filamentous fungus  Ashbya gossypii , a natural riboflavin overproducer, excretes high amounts of inosine and guanosine nucleosides to the culture medium. Following a rational metabolic engineering approach of the  de novo  purine nucleotide biosynthetic pathway, we increased the excreted levels of inosine up to 27-fold.    Conclusions  We generated  Ashbya gossypii  strains with improved production titers of inosine and guanosine. Our results point to  Ashbya gossypii  as the first eukaryotic microorganism representing a promising candidate, susceptible to further manipulation, for industrial nucleoside fermentation.    Electronic supplementary material  The online version of this article (doi:10.1186/s12934-015-0234-4) contains supplementary material, which is available to authorized users.

Increased production of inosine and guanosine by means of metabolic engineering of the purine pathway in Ashbya gossypii