English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  Recent efforts demonstrated the potential application of cyanobacteria as a “microbial cell factory” to produce butanol directly from CO 2 . However, cyanobacteria have very low tolerance to the toxic butanol, which limits the economic viability of this renewable system.    Results  Through a long-term experimental evolution process, we achieved a 150% increase of the butanol tolerance in a model cyanobacterium  Synechocystis  sp. PCC 6803 after a continuous 94 passages for 395 days in BG11 media amended with gradually increased butanol concentration from 0.2% to 0.5% ( v / v ). To decipher the molecular mechanism responsible for the tolerance increase, we employed an integrated GC-MS and LC-MS approach to determine metabolomic profiles of the butanol-tolerant  Synechocystis  strains isolated from several stages of the evolution, and then applied PCA and WGCNA network analyses to identify the key metabolites and metabolic modules related to the increased tolerance. The results showed that unstable metabolites of 3-phosphoglyceric acid (3PG),  D -fructose 6-phosphate (F6P),  D -glucose 6-phosphate (G6P), NADPH, phosphoenolpyruvic acid (PEP),  D -ribose 5-phosphate (R5P), and stable metabolites of glycerol,  L -serine and stearic acid were differentially regulated during the evolution process, which could be related to tolerance increase to butanol in  Synechocystis .    Conclusions  The study provided the first time-series description of the metabolomic changes related to the gradual increase of butanol tolerance, and revealed a metabolomic basis important for rational tolerance engineering in  Synechocystis .    Electronic supplementary material  The online version of this article (doi:10.1186/s12934-014-0151-y) contains supplementary material, which is available to authorized users.

Metabolomic basis of laboratory evolution of butanol tolerance in photosynthetic Synechocystis sp. PCC 6803