English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  As a way to determine markers of infection or disease informing disease management, and to reveal disease-associated immune mechanisms, this study sought to measure antibody and T cell responses against key lung pathogens and to relate these to patients’ microbial colonization status, exacerbation history and lung function, in Bronchiectasis (BR) and Chronic Obstructive Pulmonary Disease (COPD).    Methods  One hundred nineteen patients with stable BR, 58 with COPD and 28 healthy volunteers were recruited and spirometry was performed. Bacterial lysates were used to measure specific antibody responses by ELISA and T cells by ELIspot. Cytokine secretion by lysate-stimulated T cells was measured by multiplex cytokine assay whilst activation phenotype was measured by flow cytometry.    Results  Typical colonization profiles were observed in BR and COPD, dominated by  P.aeruginosa, H.influenzae, S.pneumoniae  and  M.catarrhalis.  Colonization frequency was greater in BR, showing association with increased antibody responses against  P.aeruginosa  compared to COPD and HV, and with sensitivity of 73% and specificity of 95%. Interferon-gamma T cell responses against  P.aeruginosa  and  S.pneumoniae  were reduced in BR and COPD, whilst reactive T cells in BR had similar markers of homing and senescence compared to healthy volunteers. Exacerbation frequency in BR was associated with increased antibodies against  P. aeruginosa, M.catarrhalis and S.maltophilia . T cell responses against  H.influenzae  showed positive correlation with FEV 1 % ( r  = 0.201,  p  = 0.033) and negative correlation with Bronchiectasis Severity Index ( r  = − 0.287,  p  = 0.0035).    Conclusion  Our findings suggest a difference in antibody and T cell immunity in BR, with antibody being a marker of exposure and disease in BR for  P.aeruginosa ,  M.catarrhalis  and  H.influenzae,  and T cells a marker of reduced disease for  H.influenzae .    Electronic supplementary material  The online version of this article (10.1186/s12931-018-0811-2) contains supplementary material, which is available to authorized users.

Anti-bacterial antibody and T cell responses in bronchiectasis are differentially associated with lung colonization and disease