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Background  Aberrant DNA methylation as the most important reason making epigenetic silencing of genes is a main mechanism of gene inactivation in patients with colorectal cancer. In this study, we decided to identify promoter methylation status of ten genes encoding WNT negative regulators, and measure the expression of  DNMT1  enzyme in colorectal cancer samples.    Results  Aberrant methylation of  APC  gene was statistically significant associated with age over 50 (p = 0.017),  DDK3  with male (p < 0.0001),  SFRP4 ,  WIF1 , and  WNT5a  with increasing tumor stage (p = 0.004, p = 0.029, and p = 0.004),  SFRP4  and  WIF1  with tumor differentiation (p = 0.009 and p = 0.031) and  SFRP2  and  SFRP5  with histological type (p = 0.001 and p = 0.025). The increasing number of methylated genes correlated with the expression levels of the  DNMT1  mRNA.    Conclusions  The rate of gene promoter methylation of WNT pathway regulators is high in colorectal cancer cells. Hyper-methylation is associated with increased expression of the  DNMT1  enzyme.

Promoter methylation analysis of WNT/β-catenin pathway regulators and its association with expression of DNMT1 enzyme in colorectal cancer