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Background  Recent evidences indicated that shear stress is critical in orchestrating gene expression in cardiovascular disease. It is necessary to identify the mechanism of shear stress influencing gene expression in physiology and pathophysiology conditions. This paper aimed to identify candidate hub genes and its transcription factors with bioinformatics.    Methods  We analyzed microarray expression profile of GSE16706 to identify differentially expressed genes (DEGs) in low shear stress (1 dyne/cm 2 ) treated human umbilical vein endothelial cells (HUVECs) compared with static condition for 24 h.    Results  652 DEGs, including 333 up-regulated and 319 down-regulated DEGs, were screen out. Functional enrichment analysis indicated enrichment items mainly included cytokine-cytokine receptor interaction and cell cycle. Five hub genes ( CDC20 ,  CCNA2 ,  KIF11 ,  KIF2C  and  PLK1 ) and one significant module (score = 17.39) were identified through protein–protein interaction (PPI) analysis. Key transcriptional factor  FOXC1  displayed close interaction with all the hub genes via gene-transcriptional factor network. Single-gene GSEA analysis indicated that  CDC20  was linked to the G2M_CHECKPOINT pathway and cell cycle pathway.    Conclusions  By using integrated bioinformatic analysis, a new transcriptional factor and hub-genes network related to HUVECs treated with low shear stress were identified. The new regulation mechanism we discovered may be a promising potential therapeutic target for cardiovascular disease.

Bioinformatic identification of hub genes and related transcription factors in low shear stress treated endothelial cells