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Background  N-acetyltransferase 13 (NAT13) is a probable catalytic component of the ARD1A-NARG1 complex possessing alpha (N-terminal) acetyltransferase activity.    Results  In this study, a full-length complementary DNA (cDNA) encoding  Schistosoma japonicum  NAT13 (SjNAT13) was isolated from schistosome cDNAs. The 621 bp open reading frame of SjNAT13 encodes a polypeptide of 206 amino acids. Real-time PCR analysis revealed SjNAT13 expression in all tested developmental stages. Transcript levels were highest in cercariae and 21-day-old worms, and higher in male adult worms than female adult worms. The rSjNAT13 protein induced high levels of anti-rSjNAT13 IgG antibodies. In two independent immunoprotection trials, rSjNAT13 induced 24.23% and 24.47% reductions in the numbers of eggs in liver. RNA interference (RNAi) results showed that small interfering RNA (siRNA) Sj-514 significantly reduced SjNAT13 transcript levels in worms and decreased egg production in vitro.    Conclusions  Thus, rSjNAT13 might play an important role in the development and reproduction of schistosomes.    Supplementary Information  The online version contains supplementary material available at 10.1186/s12917-021-03045-y.

Characterisation and preliminary functional analysis of N-acetyltransferase 13 from Schistosoma japonicum