English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  The continuing morbidity and mortality associated with infection with malaria parasites highlights the urgent need for a vaccine. The efficacy of sub-unit vaccines tested in clinical trials in malaria-endemic areas has thus far been disappointing, sparking renewed interest in the whole parasite vaccine approach. We previously showed that a chemically attenuated whole parasite asexual blood-stage vaccine induced CD4 +  T cell-dependent protection against challenge with homologous and heterologous parasites in rodent models of malaria.    Methods  In this current study, we evaluated the immunogenicity and safety of chemically attenuated asexual blood-stage  Plasmodium falciparum  (Pf) parasites in eight malaria-naïve human volunteers. Study participants received a single dose of 3 × 10 7  Pf pRBC that had been treated in vitro with the cyclopropylpyrolloindole analogue, tafuramycin-A.    Results  We demonstrate that Pf asexual blood-stage parasites that are completely attenuated are immunogenic, safe and well tolerated in malaria-naïve volunteers. Following vaccination with a single dose, species and strain transcending  Plasmodium -specific T cell responses were induced in recipients. This included induction of  Plasmodium -specific lymphoproliferative responses, T cells secreting the parasiticidal cytokines, IFN-γ and TNF, and CD3 + CD45RO +  memory T cells. Pf-specific IgG was not detected.    Conclusions  This is the first clinical study evaluating a whole parasite blood-stage malaria vaccine. Following administration of a single dose of completely attenuated Pf asexual blood-stage parasites,  Plasmodium -specific T cell responses were induced while Pf-specific antibodies were not detected. These results support further evaluation of this chemically attenuated vaccine in humans.    Trial registration  Trial registration:  ACTRN12614000228684 . Registered 4 March 2014.    Electronic supplementary material  The online version of this article (10.1186/s12916-018-1173-9) contains supplementary material, which is available to authorized users.

bmc medicine

Vaccination with chemically attenuated Plasmodium falciparum asexual blood-stage parasites induces parasite-specific cellular immune responses in malaria-naïve volunteers: a pilot study