English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Background  The shoot meristem gives rise to new organs throughout a plant’s life by the activity of pluripotent stem cells in the meristem center. Organ initiation at the periphery of the shoot meristem is triggered by the accumulation of the phytohormone auxin at the initiation site. Loss-of-function mutants of the  ZWILLE/ARGONAUTE10/PINHEAD  ( ZLL/AGO10/PNH ) gene terminate shoot meristem stem cells late in embryogenesis and can form a leaf or a leaf-like structure instead, indicating that  AGO10  activity is required to maintain shoot meristem stem cells undifferentiated.    Results  Here, we addressed whether stem cell maintenance by  AGO10  involves regulation of auxin. We found that in  zll-1  mutants, auxin accumulation and expression of the response reporter  DR5:GFP  are elevated, and transcription of the  Auxin Response Factor 2 (ARF2)  gene is upregulated. Downregulation of  ARF2  significantly restores stem cells in  zll-1  mutants, whereas increased expression of  ARF2  enhances differentiation of stem cells in  zll-1  mutants. We further found that upregulation of the  AGO10  effector gene  REVOLUTA  restores  ARF2  expression and stem cell maintenance in  zll-1  embryos.    Conclusions  Our results indicate that maintenance of shoot meristem stem cells by  AGO10  involves negative regulation of auxin signaling and, via REV-mediated downregulation of  ARF2  expression, auxin response.    Electronic supplementary material  The online version of this article (doi:10.1186/s12915-015-0180-y) contains supplementary material, which is available to authorized users.

ZLL/AGO10 maintains shoot meristem stem cells during Arabidopsis embryogenesis by down-regulating ARF2-mediated auxin response