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Background The aim of present study was to screen the novel and promising targets of curcumin in hepatocellular carcinoma diagnosis and chemotherapy. Methods Potential targets of curcumin were screened from SwissTargetPrediction, ParmMapper and drugbank databases. Potential aberrant genes of hepatocellular carcinoma were screened from Genecards databases. Fifty paired hepatocellular carcinoma patients’ gene expression profiles from the GEO database were used to test potential targets of curcumin. Besides, GO analysis, KEGG pathway enrichment analysis and PPI network construction were used to explore the underlying mechanism of candidate hub genes. ROC analysis and Kaplan-Meier analysis were used to evaluate the diagnostic and prognostic value of candidate hub genes, respectively. Real-time PCR was used to verify the results of bioinformatics analysis. Results Bioinformatics analysis results suggested that  AURKA ,  CDK1 ,  CCNB1 ,  TOP2A ,  CYP2B6 ,  CYP2C9 , and  CYP3A4  genes served as candidate hub genes.  AURKA ,  CDK1 ,  CCNB1  and  TOP2A  were significantly upregulated and correlated with poor prognosis in hepatocellular carcinoma, AUC values of which were 95.7, 96.9, 98.1 and 96.1% respectively. There was not significant correlation between the expression of  CYP2B6  and prognosis of hepatocellular carcinoma, while  CYP2C9  and  CYP3A4  genes were significantly downregulated and correlated with poor prognosis in hepatocellular carcinoma. AUC values of  CYP2B6 ,  CYP2C9 , and  CYP3A4  were 96.0, 97.0 and 88.0% respectively. In vitro, we further confirmed that curcumin significantly downregulated the expression of  AURKA ,  CDK1 , and  TOP2A  genes, while significantly upregulated the expression of  CYP2B6 ,  CYP2C9 , and  CYP3A4  genes. Conclusions Our results provided a novel panel of  AURKA ,  CDK1 ,  TOP2A ,  CYP2C9 , and  CYP3A4  candidate genes for curcumin related chemotherapy of hepatocellular carcinoma.

Bioinformatics screening the novel and promising targets of curcumin in hepatocellular carcinoma chemotherapy and prognosis