English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Tools annual

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Presents the access of premium content as premium feature

Premium Content

Global: Zendy Plus annual

Global: Zendy Free Plan

Background    Salmonella enterica serovar  Typhimurium is a foodborne pathogen that triggers inflammatory responses in the intestines of humans and livestock.  Colla corii asini  is a traditional medicine used to treat gynecologic and chronic diseases in Korea and China. However, the antibacterial activity of  Colla corii asini  has been unknown. In this study, we investigated the antibacterial activity and effects of  Colla corii asini  extract on  Salmonella typhimurium  invasion.    Methods  To tested for antibacterial effects of  Colla corii asini  extracts, we confirmed the agar diffusion using Luria solid broth medium. Also, we determined the MIC (minimum inhibitory concentration) and the MBC (minimum bactericidal concentration) value of the  Colla corii asini  ethanol extract (CEE) by using two-fold serial dilution methods. We evaluated the expression of salmonella invasion proteins including SipA, SipB and SipC by using Western blot and qPCR at the concentration of CEE without inhibition of bacterial growth. In vitro and  vivo , we determined the inhibitory effect of invasion of  S.  typhimurium on CEE by using gentamicin assay and  S.  typhimurium - infected mice .Results  CEE significantly inhibited the growth of  Salmonella  typhimurium in an agar diffuse assay and had an MIC of 0.78 mg/ml and an MBC of 1.56 mg/ml. Additionally, CEE reduced  Salmonella  typhimurium cell invasion via the inhibition of  Salmonella  typhimurium invasion proteins, such as SipA, SipB and SipC. Furthermore, CEE significantly suppressed invasion in the small intestines (ilea) of mice injected with  Salmonella  typhimurium.    Conclusion  These findings show that  Colla corii asini  exerts antibacterial activity and suppresses  Salmonella  typhimurium invasion in vitro and in vivo. Together, these findings demonstrate that  Colla corii asini  is a potentially useful therapeutic herbal medicine for treating salmonella-mediated diseases.

Antibacterial activity and effects of Colla corii asini on Salmonella typhimurium invasion in vitro and in vivo