English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  Lichens that were used in traditional medicine for ages produce numerous secondary metabolites, however our knowledge about biological activities of substances secreted by separated bionts is scarce. The main objectives of this study were to isolate and find optimal conditions for the growth of mycelia from three common lichen-forming fungi, i.e.  Caloplaca pusilla, Protoparmeliopsis muralis  and  Xanthoria parietina  and to evaluate antibacterial and antiproliferative activities of their acetone extracts.    Methods  Agar disc diffusion and broth microdilution methods were used to test antimicrobial activity against six species of bacteria. MTT method, flow cytometry assay and DAPI staining were applied to test antiproliferative activity of selected extracts against MCF-7 (human breast adenocarcinoma), PC-3 (human prostate cancer) and HeLa (human cervix adenocarcinoma) cancer cells.    Results    P. muralis  strongly inhibited the growth of Gram-positive bacteria, i.e.  Bacillus subtilis ,  Enterococcus faecalis, Staphylococcus aureus  and  Staphylococcus epidermidis  (MICs from 6.67 to 100.00 μg mL −1 ).  X. parietina  grown on PDA and G-LBM media decreased HeLa or MCF-7 cancer cells viability with IC 50  values of about 8 μg mL −1 , while  C. pusilla  grown on G-LBM medium showed the highest potency in decreasing MCF-7 (7.29 μg mL −1 ), PC-3 (7.96 μg mL −1 ) and HeLa (6.57 μg mL −1 ) cancer cells viability. We also showed induction of apoptosis in HeLa, PC-3 and MCF-7 cell lines treated with increasing concentrations of  C. pusilla  extract.    Conclusion  We showed that selected acetone extracts demonstrated a strong antimicrobial and anticancer effects that suggests that aposymbiotically cultured lichen-forming fungi can be a source of antibacterial and antiproliferative compounds.

Antibacterial and anticancer activities of acetone extracts from in vitro cultured lichen-forming fungi