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Background  The purpose of this study is to determine the effect of a mouthwash containing  Teucriumpolium  herb on  Streptococcus mutans  in mouth.    Methods  This study was a randomized, crossover, double-blind clinical trial, where we selected 22 volunteers (dental students) randomly and we divided them into two groups. The study had two phases. In each phase, one group acted as the intervention group, while the other one was the control group. Both the intervention and control groups were given the mouthwash with and without  Teucriumpolium , respectively.  S. mutans  of saliva were measured before and after each phase to compare the effects of the mouthwashes. A three-week washout period was considered between the two phases. An independent two-sample t-test was utilized to compare the mean of  S. mutans  colonies. Additionally, we used a standard AB/BA crossover model to find the results of the treatment and the impact of carryover on the residual’s biological effects. The significance level was considered 0.05 in this experiment.    Results  There is no significant difference observed between the two groups in the number of  S. mutans  before using the mouthwashes. When the mouthwash containing  Teucriumpolium  was used, there was a significant decrease in the number of  S. mutans  colonies in both phases’ extract ( P  = 0.002).  Conclusion : The results of this study indicate the mouthwash containing aqueous extract of  Teucrium polium  can majorly reduce the colonization of  S. mutans  in human saliva.    Trial registration  Ethical issues approved by the Ethics Committee of the Rafsanjan University of Medical Sciences with the approval number of 937/9/31, IRCT code Number of  IRCT2013121815842N1  and it was approved on 06/16/2014. The study was conducted in the period of September to November 2014.

The effect of antimicrobial activity of Teucrium Polium on Oral Streptococcus Mutans: a randomized cross-over clinical trial study