English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Tools annual

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Presents the access of premium content as premium feature

Premium Content

Global: Zendy Plus annual

Global: Zendy Free Plan

Background  Gingival epithelial cells are the major population of the gingival tissue, acting as the front-line defense against microbial intrusion and regulating the homeostasis of the periodontal tissue in health and disease via NLR family pyrin domain-containing-3 (NLRP3) inflammasome, which recognizes pathogen- and danger-associated molecular patterns (PAMPs and DAMPs). The aim of this study was to determine whether the activation of NLRP3 inflammasome depends on infection with the periodontal pathogen  Porphyromonas gingivalis  ( P. gingivalis ), or stimulation with  P. gingivalis  lipopolysaccharide (LPS), and/or extracellular adenosine triphosphate (ATP).    Methods  An oral epithelial cell line was treated with  P. gingivalis ,  P. gingivalis  LPS and ATP. The gene and protein expression of NLRP3 inflammasome components were quantified by real time RT-PCR and immunoblots. Production of IL-1β and IL-18 was measured by ELISA.    Results  There was no increase in NLRP3 inflammasome gene expression after  P. gingivalis  infection unless pre-stimulated by ATP. Obvious increases of NLRP3 inflammasome gene expression was observed after  P. gingivalis  LPS stimulation, even pre-stimulated by ATP at 2 h.    Conclusions  The findings indicate that the activation of NLRP3 inflammasome does not rely on  P. gingivalis  infection, unless stimulated by  P. gingivalis  LPS and/or extracellular ATP, suggesting diverse signaling pathways are involved in the host immune response.

bmc oral health

The activation of pyrin domain-containing-3 inflammasome depends on lipopolysaccharide from Porphyromonas gingivalis and extracellular adenosine triphosphate in cultured oral epithelial cells