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Background  Arachidonic acid (ARA), which is a ω-6 polyunsaturated fatty acid, has a wide range of biological activities and is an essential component of cellular membranes in some human tissues.  Mortierella alpina  is the best strain for industrial production of ARA. To increase its yield of arachidonic acid, heavy ion beam irradiation mutagenesis of  Mortierella alpina  was carried out in combination with triclosan and octyl gallate treatment.    Results  The obtained mutant strain F-23 ultimately achieved an ARA yield of 5.26 g L − 1 , which is 3.24 times higher than that of the wild-type strain. In addition, quantitative real-time PCR confirmed that the expression levels of fatty acid synthase (FAS), Δ5-desaturase, Δ6-desaturase, and Δ9-desaturase were all significantly up-regulated in the mutant F-23 strain, especially Δ6- and Δ9-desaturase, which were up-regulated 3- and 2-fold, respectively.    Conclusions  This study confirmed a feasible mutagenesis breeding strategy for improving ARA production and provided a mutant of  Mortierella alpina  with high ARA yield.

Heavy ion mutagenesis combined with triclosan screening provides a new strategy for improving the arachidonic acid yield in Mortierella alpina