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Background  Cotton fiber, a natural fiber widely used in the textile industry, is differentiated from single cell of ovule epidermis. A large number of genes are believed to be involved in fiber formation, but so far only a few fiber genes have been isolated and functionally characterized in this developmental process. The Kinesin13 subfamily was found to play key roles during cell division and cell elongation, and was considered to be involved in the regulation of cotton fiber development.    Results  The full length of coding sequence of  GhKIS13A1  was cloned using cDNA from cotton fiber for functional characterization. Expression pattern analysis showed that  GhKIS13A1  maintained a lower expression level during cotton fiber development. Biochemical assay showed that GhKIS13A1 has microtubule binding activity and basal ATPase activity that can be activated significantly by the presence of microtubules. Overexpression of  GhKIS13A1  in  Arabidopsis  reduced leaf trichomes and the percentage of three-branch trichomes, and increased two-branch and shriveled trichomes compared to wild-type. Additionally, the expression of  GhKIS13A1  in the  Arabidopsis Kinesin-13a-1  mutant rescued the defective trichome branching pattern of the mutant, making its overall trichome branching pattern back to normal.    Conclusions  Our results suggested that  GhKIS13A1  is functionally compatible with  AtKinesin-13A  regarding their role in regulating the number and branching pattern of leaf trichomes. Given the developmental similarities between cotton fibers and  Arabidopsis  trichomes, it is speculated that  GhKIS13A1  may also be involved in the regulation of cotton fiber development.

Expression and functional analyses of a Kinesin gene GhKIS13A1 from cotton (Gossypium hirsutum) fiber