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Background  Rice sheath blight, caused by  Rhizoctonia solani  is one of the most devastating diseases of rice. It is associated with significant reduction in rice productivity worldwide. A mutant variant of mannose binding  Allium sativum  leaf agglutinin (mASAL) was previously reported to exhibit strong antifungal activity against  R. solani . In this study, the  mASAL  gene has been evaluated for its  in planta  antifungal activity in rice plants.    Results  mASAL was cloned into pCAMBIA1301 binary vector under the control of CaMV35S promoter. It was expressed in an elite indica rice cv. IR64 by employing  Agrobacterium tumefaciens -mediated transformation. Molecular analyses of transgenic plants confirmed the presence and stable integration of  mASAL  gene. Immunohistofluorescence analysis of various tissue sections of plant parts clearly indicated the constitutive expression of mASAL. The segregation pattern of  mASAL  transgene was observed in T 1  progenies in a 3:1 Mendelian ratio. The expression of mASAL was confirmed in T 0  and T 1  plants through western blot analysis followed by ELISA.  In planta  bioassay of transgenic lines against  R. solani  exhibited an average of 55 % reduction in sheath blight percentage disease index (PDI).    Conclusions  The present study opens up the possibility of engineering rice plants with the antifungal gene  mASAL , conferring resistance to sheath blight.

Monitoring the efficacy of mutated Allium sativum leaf lectin in transgenic rice against Rhizoctonia solani