English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  The ∆ 6 -desaturase gene, encoding a key enzyme in the biosynthesis of polyunsaturated fatty acids, has potential in pharmaceutical and nutraceutical applications.    Results  The ∆ 6 -desaturase gene has been isolated from a selected strain of Oomycetes,  Pythium  sp. BCC53698. The cloned gene ( PyDes6 ) contained an open reading frame (ORF) of 1401 bp encoding 466 amino acid residues. The deduced amino acid sequence shared a high similarity to those of other ∆ 6 -desaturases that contained the signature features of a membrane-bound ∆ 6 -desaturase, including a cytochrome  b  5  and three histidine-rich motifs and membrane-spanning regions. Heterologous expression in  Saccharomyces cerevisiae  showed that monoene, diene and triene fatty acids having ∆ 9 -double bond were substrates for PyDes6. No distinct preference between the  n- 3 and  n -6 polyunsaturated fatty acyl substrates was found. The ∆ 6 -desaturated products were markedly increased by codon optimization of  PyDes6 .    Conclusion  The codon-optimized ∆ 6 -desaturase gene generated in this study is a promising tool for further reconstitution of the fatty acid profile, in a host system of choice, for the production of economically important fatty acids, particularly the  n- 3 and  n -6 polyunsaturated fatty acids.    Electronic supplementary material  The online version of this article (doi:10.1186/s12896-015-0200-6) contains supplementary material, which is available to authorized users.

The codon-optimized Δ6-desaturase gene of Pythium sp. as an empowering tool for engineering n3/n6 polyunsaturated fatty acid biosynthesis