English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  Labral tear can be the initiating factor in the onset of hip osteoarthritis (HOA). However, the physiopathology of labral tear is not fully understood. Our aim was to compare synovial tissue inflammatory cytokine levels between patients with labral tear and late-stage HOA.    Methods  Synovial tissue from sites showing the greatest inflammation was harvested from 106 hips from 100 subjects during hip surgery. RNA was extracted, and levels of  TNFA ,  IL1B ,  IL6  and  COX2  mRNA were compared among all patients using real-time PCR. Additionally, we examined whether femoroacetabular impingement (FAI) was associated with elevated levels of inflammatory cytokines in patients with labral tear. To analyze the effects of TNF-α on inflammatory mediators in hip synovial tissue, synovial fibroblasts were extracted from hip synovial tissue of patients with labral tear and late-stage HOA ( n  = 5 each). Mononuclear cells were extracted from synovial tissue, cultured for 7 days, and stimulated with control or 10 ng/mL human recombinant TNF-α for 1 day. mRNA was extracted from stimulated cells and  IL1B ,  IL6 , and  COX2  levels were determined using real-time PCR.    Results   TNFA ,  IL1B,  and  COX2  expression in synovial tissue were significantly higher in patients with labral tear than late-stage HOA ( TNFA ,  p  <  0.001;  IL1B ,  p  <  0.001;  COX2 ,  p  = 0.001). There were no differences in expression between patients with labral tear with and without FAI ( TNFA ,  p  = 0.546;  IL1B ,  p  = 0.559;  IL6 ,  p  = 0.599;  COX2 ,  p  = 0.124). Compared to vehicle control, TNF-α stimulation significantly elevated  IL1B ,  IL6,  and  COX2  expression in synovial fibroblasts collected from patients with labral tear and late-stage HOA ( IL1B ,  p  = 0.043 and  p  = 0.043;  IL6 ,  p  = 0.043 and 0.043;  COX2 ,  p  = 0.043 and  p  = 0.080, respectively).    Conclusions   TNFA ,  IL1B,  and  COX2  expression were elevated in the synovial tissue of patients with labral tear. Further investigations are needed to reveal the relationship between inflammatory cytokine levels and various aspects of labral tear pathology, including pain and the onset and progression of OA.    Supplementary Information  The online version contains supplementary material available at 10.1186/s12891-020-03888-w.

Elevated levels of TNF-α, IL-1β and IL-6 in the synovial tissue of patients with labral tear: a comparative study with hip osteoarthritis