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Background  Hsf4 is closely related to the development of cataract. However, the molecular mechanisms remain unknown. This study aimed to explore the molecular mechanisms that how Hsf4 mutations influence development of lens and thus lead to cataract in mouse.    Methods  The mRNA expression profile of mouse tissue samples from  Hsf4 -null and wile-type lenses was downloaded from Gene Expression Omnibus database. Then the LIMMA package was used to screen differentially expressed genes (DEGs) and DAVID was applied to identify the significantly enriched Gene Ontology (GO) categories for DEGs. Furthermore, the protein-protein interaction (PPI) network of DEGs was constructed using Cytoscape and the key modules were selected from the PPI network based on the MCODE analysis.    Results  A total of 216 DEGs were screened, including 51 up- and 165 down-regulated genes. Meanwhile, nine GO terms were obtained, and DEGs such as  SGK1 ,  CRY2  and  REV1  were enriched in response to DNA damage stimulus. Furthermore, 89 DEGs and 99 gene pairs were mapped into the PPI network and Ubc was the hob node. Two key modules, which contained the genes (e.g.  Ubc ,  Egr1 ,  Ptgs2 ,  Hmox1 ,  Cd44 ,  Btg2 ,  Cyr61  and  Fos ) were related to response to DNA damage stimulus.    Conclusions  The deletion of Hsf4 affects the expression of many genes, such as  Ubc ,  Ptgs2 ,  Egr1  and  Fos . These genes may be involved in the development of cataract and could be used as therapeutic targets for cataract.

Screening of potential target genes for cataract by analyzing mRNA expression profile of mouse Hsf4-null lens