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Virtual reality (VR) enables data visualization in an immersive and engagingmanner, and it can be used for creating ways to explore scientific data. Here,we use VR for visualization of 3D histology data, creating a novel interfacefor digital pathology. Our contribution includes 3D modeling of a whole organand embedded objects of interest, fusing the models with associatedquantitative features and full resolution serial section patches, andimplementing the virtual reality application. Our VR application is multi-scalein nature, covering two object levels representing different ranges of detail,namely organ level and sub-organ level. In addition, the application includesseveral data layers, including the measured histology image layer and multiplerepresentations of quantitative features computed from the histology. In thisinteractive VR application, the user can set visualization properties, selectdifferent samples and features, and interact with various objects. In thiswork, we used whole mouse prostates (organ level) with prostate cancer tumors(sub-organ objects of interest) as example cases, and included quantitativehistological features relevant for tumor biology in the VR model. Due toautomated processing of the histology data, our application can be easilyadopted to visualize other organs and pathologies from various origins. Ourapplication enables a novel way for exploration of high-resolution,multidimensional data for biomedical research purposes, and can also be used inteaching and researcher training.

Virtual reality for 3D histology: multi-scale visualization of organs with interactive feature exploration