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Background  microRNAs (miRNAs) play essential roles in the development and progression of gastric cancer (GC). Although aberrant  miR-874  expression has been reported in various human cancers, its role in GC remains obscure.    Methods   miR-874  expression was assessed by real-time quantitative polymerase chain reaction (RT-qPCR) in 62 matched GC and adjacent normal tissues, as well as in GC cell lines and immortalized human gastric epithelial cells. CCK8 assay, colony formation assay, and flow cytometry were used to assess the role of miR-874 in GC cell proliferation and apoptosis in vitro. Additionally, to determine the effects of  miR-874  on GC cell proliferation and apoptosis in vivo, BALB/c nude mice were injected with GC cells transfected with a  miR-874  mimic. The role of  miR-874  in  SPAG9  expression was assessed by luciferase assay, Western blotting, and RT-qPCR.    Results   miR-874  was downregulated in GC cell lines and tissues.  miR-874  overexpression in GC cells led to inhibition of cell proliferation and induction of apoptosis. Moreover,  SPAG9  was identified as a direct  miR-874  target, the expression of which was suppressed by  miR-874 .  SPAG9  overexpression markedly promoted GC cell proliferation.    Conclusions   miR-874  inhibited cell proliferation and induced apoptosis in GC cells.  SPAG9  downregulation was crucial for the tumor-suppressive effects of  miR-874 . Hence, the  miR-874 / SPAG9  axis could serve as a novel therapeutic target in GC.

miR-874 inhibits gastric cancer cell proliferation by targeting SPAG9