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Background  Y-box binding protein-1 is an evolutionary conserved transcription and translation regulating protein that is overexpressed in various human malignancies, including breast cancer. Despite reports of YB-1 and its association with distant spread of breast cancer, the intrinsic mechanism underlying this observation remains elusive. This study investigates the role of YB-1 in mediating metastasis in highly invasive breast cancer cell lines.    Methods  Silencing the  YBX1  gene (which encodes the YB-1 protein) by small interfering RNA (siRNA) was performed in MDA-MB-231 and Hs578T breast cancer cell lines, followed by phenotypic assays including cell migration and invasion assays. Gene expression profiling using Affymetrix GeneChip® Human Transcriptome 2.0 array was subsequently carried out in YB-1 silenced MDA-MB-231 cells. Overexpression and silencing of  YBX1  were performed to assess the expression of  CORO1C , one of the differentially regulated genes from the transcriptomic analysis. A Gaussia luciferase reporter assay was used to determine if  CORO1C  is a putative YB-1 downstream target. siRNA-mediated silencing of  CORO1C  and down-regulation of  YBX1  in  CORO1C  overexpressing MDA-MB-231 cells were performed to evaluate cell migration and invasion.    Results  Downregulation of the YB-1 protein inhibited cell migration and invasion in MDA-MB-231 breast cancer cells. Global gene expression profiling in the  YBX1  silenced MDA-MB-231 cells identified differential expression of several genes, including  CORO1C  (which encodes for an actin binding protein, coronin-1C) as a potential downstream target of YB-1. While knockdown of  YBX1  gene decreased  CORO1C  gene expression, the opposite effects were seen in YB-1 overexpressing cells. Subsequent verification using the reporter assay revealed that  CORO1C  is an indirect downstream target of YB-1. Silencing of  CORO1C  by siRNA in MDA-MB-231 cells was also observed to reduce cell migration and invasion. Silencing of  YBX1  caused a similar reduction in  CORO1C  expression ,  concomitant with a significant decrease in migration in Hs578T cells. In coronin-1C overexpressing MDA-MB-231 cells, increased migration and invasion were abrogated by YB-1 knockdown.    Conclusion  It would appear that YB-1 could regulate cell invasion and migration  via  downregulation of its indirect target coronin-1C. The association between YB-1 and coronin-1C offers a novel approach by which metastasis of breast cancer cells could be targeted and abrogated.    Electronic supplementary material  The online version of this article (doi:10.1186/s12885-017-3187-7) contains supplementary material, which is available to authorized users.

YBX1 gene silencing inhibits migratory and invasive potential via CORO1C in breast cancer in vitro