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Background  G proteins are known to modulate various growth signals and are implicated in the regulation of tumorigenesis. The tumor suppressor Fhit is a newly identified interaction partner of G q  proteins that typically stimulate the phospholipase C pathway. Activated Gα q  subunits have been shown to interact directly with Fhit, up-regulate Fhit expression and enhance its suppressive effect on cell growth and migration. Other signaling molecules may be involved in modulating Gα q /Fhit interaction.    Methods  To test the relationship of PLCβ with the interaction between Gα q  and Fhit, co-immunoprecipication assay was performed on HEK293 cells co-transfected with different combinations of Flag-Fhit, Gα 16 , Gα 16 QL, pcDNA3 vector, and PLCβ isoforms. Possible associations of Fhit with other effectors of Gα q  were also demonstrated by co-immunoprecipitation. The regions of Gα q  for Fhit interaction and PLCβ stimulation were further evaluated by inositol phosphates accumulation assay using a series of Gα 16/z  chimeras with discrete regions of Gα 16  replaced by those of Gα z .    Results  PLCβ1, 2 and 3 interacted with Fhit regardless of the expression of Gα q . Expression of PLCβ increased the affinities of Fhit for both wild-type and activated Gα q . Swapping of the Fhit-interacting α2-β4 region of Gα q  with Gα i  eliminated the association of Gα q  with Fhit without affecting the ability of the mutant to stimulate PLCβ. Other effectors of Gα q  including RGS2 and p63RhoGEF were unable to interact with Fhit.    Conclusions  PLCβ may participate in the regulation of Fhit by G q  in a unique way. PLCβ interacts with Fhit and increases the interaction between Gα q  and Fhit. The Gα q /PLCβ/Fhit complex formation points to a novel signaling pathway that may negatively regulate tumor cell growth.    Electronic supplementary material  The online version of this article (doi:10.1186/s12885-015-1802-z) contains supplementary material, which is available to authorized users.

Association of activated Gαq to the tumor suppressor Fhit is enhanced by phospholipase Cβ