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Background   Campylobacter jejuni  is a leading cause of bacterial diarrhea worldwide, and increasing rates of fluoroquinolone (FQ) resistance in  C. jejuni  are a major public health concern. The rapid detection and tracking of FQ resistance are critical needs in developing countries, as these antimicrobials are widely used against  C .  jejuni  infections. Detection of point mutations at T86I in the  gyrA  gene by real-time polymerase chain reaction (RT-PCR) is a rapid detection tool that may improve FQ resistance tracking.    Methods   C. jejuni  isolates obtained from children with diarrhea in Peru were tested by RT-PCR to detect point mutations at T86I in  gyrA . Further confirmation was performed by sequencing of the  gyrA  gene.    Results  We detected point mutations at T86I in the  gyrA  gene in 100% (141/141) of  C. jejuni  clinical isolates that were previously confirmed as ciprofloxacin-resistant by E-test. No mutations were detected at T86I in  gyrA  in any ciprofloxacin-sensitive isolates.    Conclusions  Detection of T86I mutations in  C .  jejuni  is a rapid, sensitive, and specific method to identify fluoroquinolone resistance in Peru. This detection approach could be broadly employed in epidemiologic surveillance, therefore reducing time and cost in regions with limited resources.

Validation of the T86I mutation in the gyrA gene as a highly reliable real time PCR target to detect Fluoroquinolone-resistant Campylobacter jejuni