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Background  Trichomoniasis resulting from  Trichomonas vaginalis  ( T. vaginalis ) has been considered as a commonly seen disease with the transmission way of sex. At present, the detection methods of  T. vaginalis  mainly include wet mount microscopy, culture, PCR, immunofluorescence and ELISA. However, all of these detection methods exist shortcomings.    Methods  In this study, a loop-mediated isothermal amplification (LAMP) assay that targeted the species-specific sequence of adhesion protein 65 (AP65) gene had been conducted to detect  T. vaginalis . The optimum reaction system and conditions were optimized in this rapid detection method.    Results  The results of sensitivity analysis showed that the LAMP assay targeting the AP65 gene was 1000 times more sensitive than the nested PCR targeting the actin gene commonly used for detection of  T. vaginalis , and the detecting limitation of the former was 10 trichomonad. Moreover, the amplification of the target gene AP65 by LAMP assay exhibited high specificity and the product was exclusively from  T. vaginalis . The detection technique of LAMP did not exhibit cross-reactivity with the common pathogens of  Trichinella spiralis ,  Toxoplasma gondii ,  Escherichia coli ,  Candida albicans ,  Staphylococcus aureus ,  Haemophilus .    Conclusions  According to the present study, the LAMP assay with the target of AP65 gene, was suitable for the early diagnosis of  T. vaginalis  infections. Consequently, the LAMP assay was proposed by the current study as a point-of-care examination and an alternative molecular tool which exhibited the potential value in the treatment, control and prevention of trichomoniasis transmission and relevant complication.

Development of a convenient detection method for Trichomonas vaginalis based on loop-mediated isothermal amplification targeting adhesion protein 65