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Background   Toxoplasma gondii  is an opportunistic protozoan parasite that can infect all warm-blooded animals including humans and cause serious clinical manifestations. Toxoplasmosis can be diagnosed using histological, serological, and molecular methods. In this study, we aimed to detect  T. gondii  RE gene in various human samples by  in house  and commercial real time polymerase chain reactions.    Methods  A total of 38 suspected cases of toxoplasmosis [peripheral blood (n:12), amnion fluid (n:11), tissue (n:9), cerebrospinal fluid (n:5), and intraocular fluid (n:1)] were included to the study. An  in house  and a commercial RT-PCR were applied to investigate the  T. gondii  RE gene in these samples.    Results  The compatibility rate of the two tests was 94.7% (37/38). When the commercial RT-PCR kit was taken as reference, the sensitivity and specificity of  in house  RT-PCR test was 87.5 and 100%. When the  in house  RT-PCR test was taken as reference, the commercial RT-PCR kit has 100% sensitivity and 96.8% specificity. Incompatibility was detected in only in a buffy coat sample with high protein content.    Conclusions  Both the commercial and  in house  RT-PCR tests can be used to investigate  T. gondii  RE gene in various clinical specimens with their high sensitivity and specificity. In house RT-PCR assay can be favorable due to cost savings compared to using the commercial test.

bmc infectious diseases

Comparison of an in house and a commercial real-time polymerase chain reaction targeting Toxoplasma gondii RE gene using various samples collected from patients in Turkey