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Background  For a long time, the Christie-Atkinson-Munch-Peterson (CAMP) test has been a standard test for the identification of  Streptococcus agalactiae , and a positive result for  S.agalactiae  has been considered sensitive enough.    Methods  To confirm whether a positive CAMP test is a requirement for the identification of  S.agalactiae , five suspected CAMP-negative  S.agalactiae  isolates from two hospitals, confirmed as Gram-positive and catalase-negative streptococci, were verified by the CAMP test in three batches of plates from two manufacturers and identified by the Phoenix system, MALDI-TOF MS, the PCR assay and the 16S rDNA gene sequencing.    Results  All five suspected strains were  S.agalactiae , four of which were CAMP-negative and one of which was not  S.agalactiae  by the PCR assay.    Conclusions  A positive CAMP test was lacking sensitivity for the identification of  S.agalactiae , and the question of whether the  cfb  gene is worthy of targeting should be further studied.

Is a positive Christie-Atkinson-Munch-Peterson (CAMP) test sensitive enough for the identification of Streptococcus agalactiae?