English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background   Acinetobacter baumannii  is a nosocomial pathogen which is reported as a major cause of morbidity and mortality in intensive care units (ICUs). However, there is a lack of analysis focused on multidrug-resistant  Acinetobacter baumannii  (MDRAB) infection among patients from pediatric intensive care unit (PICU) in China. The aim of this study was to investigate the molecular characterization of MDRAB isolated from PICU.    Methods  In this study, 86 isolates of MDRAB were collected from PICU patients, from the First Affiliated Hospital of Sun Yat-sen University. The minimal inhibitory concentrations (MICs) of the isolates against common antibiotics were determined. The carbapenemase-encoding resistance genes and  AdeABC-AdeRS  efflux system genes of these isolates were detected by PCR. Real-time PCR was performed to determine the relative expression of the relevant efflux pumps.    Results  Among 86 strains of MDRAB, 76.7% (66/86) were carbapenem-resistant  A. baumannii  (CRAB). All 86 clinical isolates possessed the  bla   OXA-51   gene.  Bla   OXA-23   was detected as the second most frequent (90.7%) carbapenemase. Harboring  AdeABC  efflux pump genes was prevalent among the majority of the MDR isolates. Specially, the distributions of  AdeABC-AdeRS  efflux system genes in CRAB strains reached up to 90.0%. Compared with those of the CSAB strains, there was a statistically significant increasing distribution of the regulator  AdeR  and  AdeS  genes( p < 0.05 ). Moreover, CRAB strains showed significantly increased expression of  AdeB (12.3- fold), but decreased expression of  AdeR  (3.3- fold)( p < 0.05 ).    Conclusion  The present study showed a high distribution of multiple genes, mainly the genes of  bla   OXA-23   /bla   OXA-51   carbapenemase and  AdeABC  efflux pump, is responsible to distinct drug-resistance in PICU. It is urgent to strengthen the molecular epidemiological surveillance of pediatric MDRAB isolates to prevent further outbreaks. This study is of significant help for the clinicians to make therapeutic decisions and manage infection control in PICU.

bmc infectious diseases

Molecular characterization of multidrug resistant strains of Acinetobacter baumannii isolated from pediatric intensive care unit in a Chinese tertiary hospital