English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  Pulmonary nocardiosis mimic pulmonary tuberculosis in most clinical and radiological manifestations. In Tanzania, where tuberculosis is one of the major public health threat clinical impact of nocardiosis as the cause of the human disease remains unknown. The objective of the present study was to isolate and identify  Nocardia  isolates recovered from TB suspects in Northeastern, Tanzania by using biochemical and molecular methods.    Methods  The study involved 744 sputum samples collected from 372 TB suspects from four periphery diagnostic centers in Northeastern, Tanzania. Twenty patients were diagnosed as having presumptively  Nocardia  infections based on microscopic, cultural characteristics and biomèrieux ID 32C Yeast Identification system and confirmed using 16S rRNA and  hsp 65 gene specific primers for  Nocardia  species and sequencing.    Results  Biochemically, the majority of the isolates were  N. asteroides  ( n  = 8/20, 40%),  N. brasiliensis  ( n  = 4/20, 20%),  N. farcinica  ( n  = 3/20, 15%),  N. nova  ( n  = 1/20, 5%). Other aerobic actinomycetales included  Streptomyces cyanescens  ( n  = 2/20, 10%),  Streptomyces griseus ,  Actinomadura madurae  each ( n  = 1/20, 5%). Results of 16S rRNA and  hsp 65 sequencing were concordant in 15/17 (88. 2%) isolates and discordant in 2/17 (11.8%) isolates. Majority of the isolates belonged to  N. cyriacigeorgica  and  N. farcinica,  four (23.5%) each.    Conclusions  Our findings suggest that  Nocardia  species may be an important cause of pulmonary nocardiosis that is underdiagnosed or ignored. This underscores needs to consider pulmonary nocardiosis as a differential diagnosis when there is a failure of anti-TB therapy and as a possible cause of human infections.    Electronic supplementary material  The online version of this article (doi:10.1186/s12879-017-2520-8) contains supplementary material, which is available to authorized users.

Isolation, biochemical and molecular identification of Nocardia species among TB suspects in northeastern, Tanzania; a forgotten or neglected threat?