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Background  Healthy condition and chronic diseases may be associated with microbiota composition and its properties. The prevalence of respiratory haemophili with respect to their phenotypes including the ability to biofilm formation in patients with sarcoidosis was assayed.    Methods  Nasopharynx and sputum specimens were taken in 31 patients with sarcoidosis (average age 42.6 ± 13), and nasopharynx specimens were taken in 37 healthy people (average age 44.6 ± 11.6). Haemophili were identified by API-NH microtest and by the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) system. Biofilm was visualised by crystal violet staining and confocal scanning laser microscopy (CSLM). The statistical analysis was performed with Statgraphics Plus for Windows.    Results  In total, 30/31 patients with sarcoidosis and 31/37 healthy people were colonized by  Haemophilus influenzae  (6/30 vs. 1/31) and  Haemophilus parainfluenzae  (28/30 vs. 31/31) in the nasopharynx. The overall number of nasopharyngeal haemophili isolates was 59 in patients with sarcoidosis and 67 in healthy volunteers ( H. influenzae  6/59 vs. 1/67,  P =  0.05;  H. parainfluenzae  47/59 vs. 65/67,  P =  0.0032). Moreover, the decreased number of  H. parainfluenzae  biofilm-producing isolates was shown in nasopharyngeal samples in patients with sarcoidosis as compared to healthy people (19/31 vs. 57/65,  P =  0.006), especially with respect to isolates classified as strong and very strong biofilm-producers (8/31 vs. 39/65,  P =  0.002).    Conclusions  The obtained data suggest that the qualitative and quantitative changes within the respiratory microbiota concerning the overall prevalence of  H. influenzae  together with the decreased number of  H. parainfluenzae  strains and the decreased rate of  H. parainfluenzae  biofilm-producing isolates as compared to healthy people may be associated with sarcoidosis.

bmc infectious diseases

Changes in the prevalence and biofilm formation of Haemophilus influenzae and Haemophilus parainfluenzae from the respiratory microbiota of patients with sarcoidosis