English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  Recently, we introduced a novel peptide nucleic acid (PNA) multi-probe real time PCR method targeting the  hsp65  gene ( hsp65  PNA RT-PCR) to distinguish  Mycobacterium abscessus  groups.    Methods  Here, we evaluated the usefulness of the  hsp65  PNA RT-PCR for the direct identification of the  M. abscessus  group at the subspecies and genotype levels from sputa samples. The method was applied to total sputa DNA from 60 different patients who were identified as having mycobacterial infections via  rpoB  PCR restriction analysis of the same cultures.    Results  The  hsp65  PNA RT-PCR method had higher sensitivity than the multi-probe real-time PCR assay targeting  hsp65  (HMPRT-PCR) for the detection of  M. abscessus  from sputum [96.7 % (29/30 samples) vs. 70 % (21/30 samples); 100 % specificity].    Conclusions  These results suggest that the PNA-based method is feasible for the detection of  M. abscessus  members not only from cultures but also directly from sputa.    Electronic supplementary material  The online version of this article (doi:10.1186/s12879-015-1076-8) contains supplementary material, which is available to authorized users.

bmc infectious diseases

Separation of Mycobacterium abscessus into subspecies or genotype level by direct application of peptide nucleic acid multi-probe- real-time PCR method into sputa samples