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Background  In mammals, the IGF-1 pathway affects the phenotype of aging. Since the function of the immune system is modulated by IGF-1, it is plausible that immunosenescence might in part result from altered control by this pathway. We therefore examined whether the expression of  IGF-1R ,  FOXO1 , and  FOXO3a  in peripheral blood mononuclear cells (PBMC) changes with age and if this might be due to changes in the expression of select miRNAs.    Methods  The expression of  IGF-1R ,  FOXO1 ,  FOXO3a , as well as of miR-9, miR-96, miR-99a, miR-132, miR-145, and miR-182 was examined in PBMC of young (27.8 ± 3.7 years), elderly (65.6 ± 3.4 years), and long-lived (94.0 ± 3.7 years) Polish Caucasians using real-time PCR. mRNA/miRNA interactions were studied in HEK 293 cells using luciferase-expressing pmirGLO reporter vector.    Results  The median expression of  IGF-1R  decreased with age ( p  < 0.1), as did the expression of  FOXO1  ( p  < 0.1), while the expression of  FOXO3a  remained stable. We also found an age-associated increase of the median expression of miR-96 ( p  = 0.002), miR-145 ( p  = 0.024) and miR-9 ( p  = 0.026), decrease of the expression of miR-99a ( p  = 0.037), and no changes regarding miR-132 and miR-182. Functional studies revealed that miR-96 and miR-182 interacted with human  IGF-1R  mRNA, and that miR-145 and miR-132 interacted with human  FOXO1  mRNA.    Conclusions  The age-associated higher expression of miR-96 and miR-145 might contribute to the lower expression of  IGF-1R  while the higher expression of miR-96, miR-145 and miR-9 might contribute to the lower expression of  FOXO1  in peripheral blood mononuclear cells of aging humans. Sustained expression/function of  FOXO3a  but not of the other two genes might be important for the maintenance of the immune system function in these individuals.

miR-96, miR-145 and miR-9 expression increases, and IGF-1R and FOXO1 expression decreases in peripheral blood mononuclear cells of aging humans