English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  Cysteine-rich receptor-like kinases (CRKs) represent a large subfamily of receptor-like kinases and play vital roles in diverse physiological processes in regulating plant growth and development.    Results   CaCRK5  transcripts were induced in pepper upon the infection of  Ralstonia solanacearum  and treatment with salicylic acid. The fusions between CaCRK5 and green fluorescence protein were targeted to the plasma membrane. Suppression of  CaCRK5  via virus-induced gene silencing (VIGS) made pepper plants significantly susceptible to  R. solanacearum  infection, which was accompanied with decreased expression of defense related genes  CaPR1 ,  CaSAR8.2 ,  CaDEF1  and  CaACO1 . Overexpression of  CaCRK5  increased resistance against  R. solanacearum  in  Nicotiana benthamiana . Furthermore, electrophoretic mobility shift assay and chromatin immunoprecipitation coupled with quantitative real-time PCR analysis revealed that a homeodomain zipper I protein CaHDZ27 can active the expression of  CaCRK5  through directly binding to its promoter. Yeast two-hybrid and bimolecular fluorescence complementation (BiFC) analyses suggested that CaCRK5 heterodimerized with the homologous member CaCRK6 on the plasma membrane.    Conclusions  Our data revealed that CaCRK5 played a positive role in regulating immune responses against  R. solanacearum  infection in pepper.    Supplementary Information  The online version contains supplementary material available at 10.1186/s12870-021-03150-y.

A cysteine-rich receptor-like protein kinase CaCKR5 modulates immune response against Ralstonia solanacearum infection in pepper