English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background   Canavalia rosea  (Sw.) DC. (bay bean) is an extremophile halophyte that is widely distributed in coastal areas of the tropics and subtropics. Seawater and drought tolerance in this species may be facilitated by aquaporins (AQPs), channel proteins that transport water and small molecules across cell membranes and thereby maintain cellular water homeostasis in the face of abiotic stress. In  C. rosea , AQP diversity, protein features, and their biological functions are still largely unknown.    Results  We describe the action of AQPs in  C. rosea  using evolutionary analyses coupled with promoter and expression analyses. A total of 37 AQPs were identified in the  C. rosea  genome and classified into five subgroups: 11 plasma membrane intrinsic proteins, 10 tonoplast intrinsic proteins, 11 Nod26-like intrinsic proteins, 4 small and basic intrinsic proteins, and 1 X-intrinsic protein. Analysis of RNA-Seq data and targeted qPCR revealed organ-specific expression of aquaporin genes and the involvement of some AQP members in adaptation of  C. rosea  to extreme coral reef environments. We also analyzed  C. rosea  sequences for phylogeny reconstruction, protein modeling, cellular localizations, and promoter analysis. Furthermore, one of PIP1 gene,  CrPIP1;5 , was identified as functional using a yeast expression system and transgenic overexpression in  Arabidopsis .    Conclusions  Our results indicate that AQPs play an important role in  C. rosea  responses to saline-alkaline soils and drought stress. These findings not only increase our understanding of the role AQPs play in mediating  C. rosea  adaptation to extreme environments, but also improve our knowledge of plant aquaporin evolution more generally.    Supplementary Information  The online version contains supplementary material available at 10.1186/s12870-021-03034-1.

bmc plant biology

Genome-wide identification and expression analysis of aquaporin family in Canavalia rosea and their roles in the adaptation to saline-alkaline soils and drought stress