English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  Triacylglycerols (TAGs) are the main composition of plant seed oil. Long-chain acyl-coenzyme A synthetases (LACSs) catalyze the synthesis of long-chain acyl-coenzyme A, which is one of the primary substrates for TAG synthesis. In  Arabidopsis , the LACS gene family contains nine members, among which  LACS1  and  LACS9  have overlapping functions in TAG biosynthesis. However, functional characterization of LACS proteins in rapeseed have been rarely reported.    Results  An orthologue of the  Arabidopsis LACS2  gene ( BnLACS2 ) that is highly expressed in developing seeds was identified in rapeseed ( Brassica napus ). The  Bn LACS2-GFP fusion protein was mainly localized to the endoplasmic reticulum, where TAG biosynthesis occurs. Interestingly, overexpression of the  BnLACS2  gene resulted in significantly higher oil contents in transgenic rapeseed plants compared to wild type, while  BnLACS2 -RNAi transgenic rapeseed plants had decreased oil contents. Furthermore, quantitative real-time PCR expression data revealed that the expression of several genes involved in glycolysis, as well as fatty acid (FA) and lipid biosynthesis, was also affected in transgenic plants.    Conclusions  A long chain acyl-CoA synthetase,  Bn LACS2 ,  located in the endoplasmic reticulum was identified in  B. napus . Overexpression of  BnLACS2  in yeast and rapeseed could increase oil content, while  BnLACS2 -RNAi transgenic rapeseed plants exhibited decreased oil content. Furthermore,  BnLACS2  transcription increased the expression of genes involved in glycolysis, and FA and lipid synthesis in developing seeds. These results suggested that  BnLACS2  is an important factor for seed oil production in  B. napus .

Long-chain acyl-CoA synthetase 2 is involved in seed oil production in Brassica napus