English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  The 29-member Arabidopsis  AHL  gene family is classified into three main classes based on nucleotide and protein sequence evolutionary differences. These differences include the presence or absence of introns, type and/or number of conserved AT-hook and PPC domains.  AHL  gene family members are divided into two phylogenetic clades, Clade-A and Clade-B. A majority of the 29 members remain functionally uncharacterized. Furthermore, the biological significance of the DNA and peptide sequence diversity, observed in the conserved motifs and domains found in the different AHL types, is a subject area that remains largely unexplored.    Results  Transgenic plants overexpressing  AtAHL20  flowered later than the wild type under both short and long days. Transcript accumulation analyses showed that  35S:AtAHL20  plants contained reduced  FT, TSF, AGL8  and  SPL3  mRNA levels. Similarly, overexpression of  AtAHL20’s  orthologue in  Camelina sativa,  Arabidopsis’ closely related  Brassicaceae  family member species, conferred a late-flowering phenotype via suppression of  CsFT  expression. However, overexpression of an aberrant  AtAHL20  gene harboring a missense mutation in the AT-hook domain’s highly conserved R-G-R core motif abolished the late-flowering phenotype. Data from targeted yeast-two-hybrid assays showed that AtAHL20 interacted with itself and several other Clade-A Type-I AHLs which have been previously implicated in flowering-time regulation: AtAHL19, AtAHL22 and AtAHL29.    Conclusion  We showed via gain-of-function analysis that  AtAHL20  is a negative regulator of  FT  expression, as well as other downstream flowering time regulating genes. A similar outcome in  Camelina sativa  transgenic plants overexpressing  CsAHL20  suggest that this is a conserved function. Our results demonstrate that  AtAHL20  acts as a photoperiod-independent negative regulator of transition to flowering.    Supplementary Information  The online version contains supplementary material available at 10.1186/s12870-020-02733-5.

Overexpression of AtAHL20 causes delayed flowering in Arabidopsis via repression of FT expression