English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  Sucrose phosphate synthase (SPS) genes play vital roles in sucrose production across various plant species. Modern sugarcane cultivar is derived from the hybridization between the high sugar content species  Saccharum officinarum  and the high stress tolerance species  Saccharum spontaneum , generating one of the most complex genomes among all crops. The genomics of sugarcane SPS remains under-studied despite its profound impact on sugar yield.    Results  In the present study, 8 and 6 gene sequences for SPS were identified from the BAC libraries of  S. officinarum  and  S. spontaneum , respectively. Phylogenetic analysis showed that  SPSD  was newly evolved in the lineage of Poaceae species with recently duplicated genes emerging from the  SPSA  clade. Molecular evolution analysis based on Ka/Ks ratios suggested that polyploidy reduced the selection pressure of  SPS  genes in  Saccharum  species. To explore the potential gene functions, the  SPS  expression patterns were analyzed based on RNA-seq and proteome dataset, and the sugar content was detected using metabolomics analysis. All the  SPS  members presented the trend of increasing expression in the sink-source transition along the developmental gradient of leaves, suggesting that the  SPSs  are involved in the photosynthesis in both  Saccharum s pecies as their function in dicots. Moreover,  SPSs  showed the higher expression in  S. spontaneum  and presented expressional preference between stem ( SPSA ) and leaf ( SPSB ) tissue, speculating they might be involved in the differentia of carbohydrate metabolism in these two  Saccharum  species, which required further verification from experiments.    Conclusions   SPSA  and  SPSB  genes presented relatively high expression and differential expression patterns between the two  Saccharum  species, indicating these two  SPSs  are important in the formation of regulatory networks and sucrose traits in the two  Saccharum  species.  SPSB  was suggested to be a major contributor to the sugar accumulation because it presented the highest expressional level and its expression positively correlated with sugar content. The recently duplicated  SPSD2  presented divergent expression levels between the two  Saccharum  species and the relative protein content levels were highest in stem, supporting the neofunctionalization of the  SPSD  subfamily in  Saccharum .

Comparative analysis of sucrose phosphate synthase (SPS) gene family between Saccharum officinarum and Saccharum spontaneum