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Background  NAC (NAM, ATAF and CUC) transcriptional factors constitute a large family with more than 150 members in rice and several members of this family have been demonstrated to play crucial roles in rice abiotic stress response. In the present study, we report the function of a novel stress-responsive NAC gene,  ONAC066 , in rice drought and oxidative stress tolerance.    Results  ONAC066 was localized in nuclei of cells when transiently expressed in  Nicotiana benthamiana  and is a transcription activator with the binding ability to NAC recognition sequence (NACRS) and AtJUB1 binding site (JBS). Expression of  ONAC066  was significantly induced by PEG, NaCl, H 2 O 2  and abscisic acid (ABA). Overexpression of  ONAC066  in transgenic rice improved drought and oxidative stress tolerance and increased ABA sensitivity, accompanied with decreased rate of water loss, increased contents of proline and soluble sugars, decreased accumulation of reactive oxygen species (ROS) and upregulated expression of stress-related genes under drought stress condition. By contrast, RNAi-mediated suppression of  ONAC066  attenuated drought and oxidative stress tolerance and decreased ABA sensitivity, accompanied with increased rate of water loss, decreased contents of proline and soluble sugars, elevated accumulation of ROS and downregulated expression of stress-related genes under drought stress condition. Furthermore, yeast one hybrid and chromatin immunoprecipitation-PCR analyses revealed that ONAC066 bound directly to a JBS-like  cis -elements in  OsDREB2A  promoter and activated the transcription of  OsDREB2A .    Conclusion  ONAC066 is a nucleus-localized transcription activator that can respond to multiple abiotic stress factors. Functional analyses using overexpression and RNAi-mediated suppression transgenic lines demonstrate that ONAC066 is a positive regulator of drought and oxidative stress tolerance in rice.    Electronic supplementary material  The online version of this article (10.1186/s12870-019-1883-y) contains supplementary material, which is available to authorized users.

Rice NAC transcription factor ONAC066 functions as a positive regulator of drought and oxidative stress response