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Background  The functional characteristics of  SLAC/SLAH  family members isolated from  Arabidopsis thaliana , poplar, barley and rice have been comprehensively investigated. However, there are no reports regarding  SLAC/SLAH  family genes from  Rosaceae  plants .Results  In this study, the function of PbrSLAH3, which is predominately expressed in pear ( Pyrus bretschneideri ) root, was investigated. PbrSLAH3 can rescue the ammonium toxicity phenomenon of  slah3  mutant plants under high-ammonium/low-nitrate conditions. In addition, yeast two-hybrid and bimolecular fluorescence complementation assays confirmed that PbrSLAH3 interacts with PbrCPK32. Moreover, when  PbrSLAH3  was co-expressed with either the  Arabidopsis  calcium-dependent protein kinase ( CPK )  21  or  PbrCPK32  in  Xenopus  oocytes, yellow fluorescence was emitted from the oocytes and typical anion currents were recorded in the presence of extracellular NO 3  − . However, when  PbrSLAH3  alone was injected, no yellow fluorescence or anion currents were recorded, suggesting that anion channel PbrSLAH3 activity was controlled through phosphorylation. Finally, electrophysiological and transgene results showed that PbrSLAH3 was more permeable to NO 3  −  than Cl − .    Conclusion  We suggest that PbrSLAH3 crossing-talk with PbrCPK32 probably participate in transporting of nitrate nutrition in pear root.    Electronic supplementary material  The online version of this article (10.1186/s12870-019-1813-z) contains supplementary material, which is available to authorized users.

PbrSLAH3 is a nitrate-selective anion channel which is modulated by calcium-dependent protein kinase 32 in pear