English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  Four-Coumarate:CoA ligase gene ( 4CL ) plays multiple important roles in plant growth and development by catalyzing the formation of CoA ester.  4CL  belongs to the plant phenylpropane derivative, which is related to the synthesis of flavonoids and lignin and is a key enzyme in the biosynthetic pathway.    Results  In this study, 12  4CL  genes of  Fraxinus mandschurica  were identified and named  Fm4CL1 - Fm4CL12 , respectively. The analysis of the expression pattern of  Fm4CL  genes indicate that  Fm4CL-like 1  gene may play a role in the lignin synthesis pathway. Our study indicate that overexpression of  Fm4CL-like 1  increases the lignin content of transgenic tobacco by 39.5% compared to WT, and the S/G ratio of transgenic tobacco increased by 19.7% compared with WT. The xylem cell layer of transgenic line is increased by 40% compared to WT, the xylem cell wall thickness increased by 21.6% compared to the WT. Under mannitol-simulated drought stress, the root length of transgenic tobacco is 64% longer than WT, and the seed germination rate of the transgenic lines is 47% higher than that of WT. In addition, the H 2 O 2  content in the transgenic tobacco was 22% lower than that of WT, while the POD and SOD content was higher than WT by 30 and 24% respectively, which showed  Fm4CL-like 1  affect the accumulation of reactive oxygen species (ROS). The MDA content and relative conductivity was 25 and 15% lower than WT, respectively. The water loss rate is 16.7% lower than that of WT. The relative expression levels of stress-related genes  NtHAK ,  NtAPX ,  NtCAT ,  NtABF2 , and  NtZFP  were higher than those of WT under stress treatment. The stomatal apertures of OE (Overexpression) were 30% smaller than those of WT, and the photosynthetic rate of OE was 48% higher than that of WT. These results showed that the overexpression line exhibited stronger adaptability to osmotic stress than WT.    Conclusions  Our results indicate that  Fm4CL-like 1  is involved in secondary cell wall development and lignin synthesis.  Fm4CL-like 1  play an important role in osmotic stress by affecting cell wall and stomatal development.    Electronic supplementary material  The online version of this article (10.1186/s12870-019-1812-0) contains supplementary material, which is available to authorized users.

bmc plant biology

Molecular cloning and functional analysis of 4-Coumarate:CoA ligase 4(4CL-like 1)from Fraxinus mandshurica and its role in abiotic stress tolerance and cell wall synthesis