English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Background   Lycium ruthenicum Murray  is an important economic plant in China and contains higher levels of anthocyanins in its fruits than other  Lyciums . However, the genetic mechanism of anthocyanin production in this plant is unknown.    Results  Based on previous transcriptome analysis,  LrAN2  and  LbAN2 , encoding MYB transcription factors, were isolated from  L. ruthenicum  and  L. barbarum , respectively. Both genes contained two introns, encoded 257 amino acids with two-Aa difference, and carried the unabridged HTH-MYB, MYB-like DNA-binding, and SANT domains. In the phylogenetic trees,  LrAN2  and  LbAN2  were found to be closely related to  NtAN2 , which regulates anthocyanin biosynthesis in tobacco. Overexpression of  LrAN2  and  LbAN2  induced anthocyanin biosynthesis in all tissues of tobacco. The anthocyanin content in the leaves of transgenic lines with  LbAN2  was lower than  LrAN2 . It indicated that the function of  LbAN2  was weaker than  LrAN2 . The  AN2  transcript could be detected only in the fruits of  L. ruthenicum  and increased during fruit development, accompanied by anthocyanin accumulation. In natural population, the alleles  LrAN2  and  LrAN2  were associated strictly with  L. ruthenicum  and  L. barbarum , respectively .  Moreover, an  AN2  genetic diversity study suggested that  Lyciums  with yellow, white, purple, and jujube red fruits were derived from  L. ruthenicum .    Conclusions  Two  AN2  alleles, from  L. ruthenicum  and  L. barbarum , were functional MYB transcriptor regulating anthocyanin biosynthesis .  The functional diversity and high expression level of  LrAN2  could be the reason for high anthocyanin content in the fruit of  L. ruthenicum .  Lyciums  with yellow, white, purple, and jujube red fruits were derived from  L. ruthenicum  based on  AN2  sequence diversity. The results may be advantageous in identifying new varieties and breeding new cultivars.    Electronic supplementary material  The online version of this article (10.1186/s12870-019-1752-8) contains supplementary material, which is available to authorized users.

Functional MYB transcription factor encoding gene AN2 is associated with anthocyanin biosynthesis in Lycium ruthenicum Murray