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Background  This study aimed to identify the transcription factors of nitrate reductase genes ( NIA1  and  NIA2 ) promoters and hypothetical  cis -element of NRE2. Based on the constructed cDNA library of  Nicotiana tabacum  K326, a yeast one-hybrid system was established using the Matchmaker® Gold Yeast One-Hybrid Library Screening System from Clontech. The transcription factors of  NIA1  and NIA2  promoters and NRE2  cis -elements were screened.    Results  After sequencing and bioinformatics analysis, 15 cDNA sequences were identified: 9 for  NIA1  (including XP_016503563.1 and NP_001312236.1), 3 for  NIA2  (including XP_016510250.1), and 3 for  NRE2  (including XM_016576899.1). XP_016503563.1 was annotated in PREDICTED: CRM-domain containing factor CFM3, and NP_001312236.1chloroplastic/mitochondrial-like in  Nicotiana tabacum . NP_001312236.1 was annotated in Sulfite oxidase-like of  Nicotiana tabacum . XP_016510250.1 was annotated as PREDICTED: uncharacterized protein LOC107827596 in  Nicotiana tabacum . XM_016576899.1 was annotated in PREDICTED:  Nicotiana tabacum  RING-H2 finger protein ATL16-like (LOC107759033).    Conclusion  A yeast one-hybrid library was successfully constructed. The identified transcription factors may provide a theoretical basis for the study of plant nitrate reductase.

bmc plant biology

Identification of transcription factors of nitrate reductase gene promoters and NRE2 cis-element through yeast one-hybrid screening in Nicotiana tabacum