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Background  Chrysanthemum is among the top ten traditional flowers in China, and one of the four major cut flowers in the world, but the growth of chrysanthemum is severely restricted by high temperatures which retard growth and cause defects in flowers. DREB (dehydration-responsive element-binding) transcription factors play important roles in the response to abiotic and biotic stresses. However, whether the DREB A-6 subgroup is involved in heat tolerance has not been reported conclusively.    Result  In the present study,  CmDREB6  was cloned from chrysanthemum ( Chrysanthemum morifolium ) ‘Jinba’. CmDREB6, containing a typical AP2/ERF domain, was classed into the DREB A-6 subgroup and shared highest homology with  Cichorium intybus  L.  CiDREB6  (73%).  CmDREB6  was expressed at its highest levels in the leaf. The CmDREB6 protein localized to the nucleus. Based on the yeast one hybrid assay,  CmDREB6  showed transcription activation activity in yeast, and the transcriptional activation domain was located in the 3 ‘end ranging from 230 to 289 amino acids residues.  CmDREB6  overexpression enhanced the tolerance of chrysanthemum to heat. The survival rate of two transgenic lines was as high as 85%, 50%, respectively, in contrast to 3.8% of wild-type (WT). Over-expression of  CmDREB6  promoted the expression of  CmHsfA4 ,  CmHSP90,  and the active oxygen scavenging genes  CmSOD  and  CmCAT .    Conclusion  In this study, DREB A-6 subgroup gene  CmDREB6  was cloned from chrysanthemum ‘Jinba’. Overexpression of  CmDREB6  enhanced heat tolerance of chrysanthemum by regulating genes involved in the heat shock response and ROS homeogenesis.    Electronic supplementary material  The online version of this article (10.1186/s12870-018-1400-8) contains supplementary material, which is available to authorized users.

Over-expression of chrysanthemum CmDREB6 enhanced tolerance of chrysanthemum to heat stress