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Background  Plant TRANSPARENT TESTA GLABRA (TTG) proteins regulate various developmental activities via the auxin signaling pathway. Recently, we elucidated the developmental role of tobacco ( Nicotiana tabacum  L.) NtTTG2 in association with 12 genes that putatively encode AUXIN RESPONSIVE FACTOR (ARF) proteins, including NtARF8, NtARF17, and NtARF19. Here we show that NtTTG2 regulates tobacco growth and development by involving the  NtARF8 ,  NtARF17 , and  NtARF19  genes, with the  NtARF8  gene playing a predominant contribution.    Results  Independent silencing of the  NtARF8  gene more strongly repressed tobacco growth than silencing the  NtARF17  or  NtARF19  gene and more effectively eradicated the growth enhancement effect of  NtTTG2  overexpression. In contrast, plant growth was not affected by silencing additional nine  NtTTG2 -regulated  NtARF  genes. In double and triple gene silencing combinations, silencing the  NtARF8  gene was more effective than silencing the  NtARF17  or  NtARF19  gene to repress growth as well as nullify growth enhancement. Therefore, the  NtARF8  predominantly cooperated with the  NtARF17  and  NtAFR19  of the  NtTTG2  functional pathway.  NtARF8  also contributed to NtTTG2-regulated seed production as concurrent  NtTTG2  and  NtARF8  overexpression played a synergistic role in seed production quantity, whereas concurrent silencing of both genes caused more severe seed abortion than single gene silencing. In plant cells, the NtTTG2 protein facilitated the nuclear import of NtARF8 as well as increased its function as a transcription activator.    Conclusions  NtARF8 is an integral component of the NtTTG2 functional pathway, which regulates tobacco growth and development.    Electronic supplementary material  The online version of this article (doi:10.1186/s12870-016-0815-3) contains supplementary material, which is available to authorized users.

Tobacco TTG2 regulates vegetative growth and seed production via the predominant role of ARF8 in cooperation with ARF17 and ARF19