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Background  We studied whether regulation of  Cdca7  ( Cell division cycle associated 7 ) expression by transcription factor Pax6 contributes to Pax6’s cellular actions during corticogenesis. The function of Cdca7 in mediating Pax6’s effects during corticogenesis has not been explored. Pax6 is expressed by radial glial progenitors in the ventricular zone of the embryonic cortical neuroepithelium, where it is required for the development of a normal complement of Tbr2-expressing intermediate progenitor cells in the subventricular zone. Pax6’s expression levels are graded across the ventricular zone, with highest levels laterally where Tbr2-expressing progenitors are generated in greatest numbers at early stages of corticogenesis.    Methods  We used in situ hybridization and immunohistochemistry to analyse patterns of  Cdca7  and  Pax6  expression in cortical tissue from wild-type and  Pax6   − / −  embryos. In each genotype we compared the graded expression of the two genes quantitatively at several ages. To test whether defects in Cdca7 expression in lateral cortical cells might contribute to the cellular defects in this region caused by Pax6 loss, we electroporated a Cdca7 expression vector into wild-type lateral cortex and examined the effect on the production of Tbr2-expressing cells.    Results  We found that  Cdca7  is co-expressed with  Pax6  in cortical progenitors, at levels opposite to those of  Pax6 . Lowest levels of  Cdca7  are found in the radial glial progenitors of lateral cortex, where Pax6 levels are highest. Higher levels of Cdca7 are found in ventral telencephalon, where Pax6 levels are low. Loss of Pax6 causes  Cdca7  expression to increase in the lateral cortex. Elevating Cdca7 in normal lateral cortical progenitors to levels close to those normally found in ventral telencephalon reduces their production of Tbr2-expressing cells early in lateral cortical formation.    Conclusion  Our results suggest that Pax6 normally represses  Cdca7  expression in the lateral cortex and that repression of  Cdca7  in cells of this region is required for their production of a normal complement of Tbr2-expressing intermediate progenitors.

Lateral cortical Cdca7 expression levels are regulated by Pax6 and influence the production of intermediate progenitors