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Background  The polyketide antibiotic 2,4-diacetylphloroglucinol (2,4-DAPG), produced by  Pseudomonas fluorescens  2P24, is positively regulated by the GacS-GacA two-component system.    Results  Here we reported on the characterization of DsbA1 (disulfide oxidoreductase) as novel regulator of biocontrol activity in  P. fluorescens . Our data showed that mutation of  dsbA1  caused the accumulation of 2,4-DAPG in a GacA-independent manner. Further analysis indicated that DsbA1 interacts with membrane-bound glucose dehydrogenase Gcd, which positively regulates the production of 2,4-DAPG. Mutation of cysteine (C)-235, C275, and C578 of Gcd, significantly reduced the interaction with DsbA1, enhanced the activity of Gcd and increased 2,4-DAPG production.    Conclusions  Our results suggest that DsbA1 regulates the 2,4-DAPG concentration via fine-tuning the function of Gcd in  P. fluorescens  2P24.

bmc microbiology

The Oxidoreductase DsbA1 negatively influences 2,4-diacetylphloroglucinol biosynthesis by interfering the function of Gcd in Pseudomonas fluorescens 2P24