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Background  Zinc uptake-regulator (Zur)-regulated lipoprotein A (ZrlA) plays a role in bacterial fitness and overcoming antimicrobial exposure in  Acinetobacter baumannii . This study further characterized the  zrlA  gene and its encoded protein and investigated the roles of the  zrlA  gene in bacterial morphology, antimicrobial susceptibility, and production of outer membrane vesicles (OMVs) in  A. baumannii  ATCC 17978.    Results  In silico and polymerase chain reaction analyses showed that the  zrlA  gene was conserved among  A. baumannii  strains with 97–100% sequence homology. Recombinant ZrlA protein exhibited a specific enzymatic activity of D-alanine-D-alanine carboxypeptidase. Wild-type  A. baumannii  exhibited more morphological heterogeneity than a Δ zrlA  mutant strain during stationary phase. The Δ zrlA  mutant strain was more susceptible to gentamicin than the wild-type strain. Sizes and protein profiles of OMVs were similar between the wild-type and Δ zrlA  mutant strains, but the Δ zrlA  mutant strain produced 9.7 times more OMV particles than the wild-type strain. OMVs from the Δ zrlA  mutant were more cytotoxic in cultured epithelial cells than OMVs from the wild-type strain.    Conclusions  The present study demonstrated that  A. baumannii  ZrlA contributes to bacterial morphogenesis and antimicrobial resistance, but its deletion increases OMV production and OMV-mediated host cell cytotoxicity.    Supplementary Information  The online version contains supplementary material available at 10.1186/s12866-020-02083-0.

bmc microbiology

The role of Zur-regulated lipoprotein A in bacterial morphology, antimicrobial susceptibility, and production of outer membrane vesicles in Acinetobacter baumannii